Part:BBa_K1725422:Design

J23101 + RBS + UirR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 206
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 510
  

Design Notes

The regulator was ligated to J23101 (which came in the form of an annealed oligo) after restriction that was followed by an Azure-A-based gel extraction.

Source

The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.